Antibody Labeling Kits
Any antibody. Any color. Any time.
FlexAble系列是Proteintech研发的一种新型抗体标记试剂盒，采用独有的FlexLinker®抗体标记技术专利，能在任何抗体缓冲液条件下，快速将荧光染料、酶、以及其他分子与抗体结合。
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与任何品牌一抗都兼容
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单次最少可标记0.5ug抗体
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适用于任何抗体浓度和配方
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标记过程中抗体无损失
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两步法10分钟快速标记
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无需额外设备或更换缓冲液
FlexAble系列是Proteintech研发的一种新型抗体标记试剂盒，采用独有的FlexLinker®抗体标记技术专利，能在任何抗体缓冲液条件下，快速将荧光染料、酶、以及其他分子与抗体结合。
-
与任何品牌一抗都兼容
-
适用于任何抗体浓度和配方
-
两步法10分钟快速标记
-
单次最少可标记0.5ug抗体
-
标记过程中抗体无损失
-
无需额外设备或更换缓冲液
Immunofluorescence of human kidney: FFPE human kidney sections were stained with anti-Calbindin antibody (14479-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, yellow), anti-ACE2 antibody (66699-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta), CoraLite®488-conjugated Podocalyxin antibody (CL488-18150, green), and DAPI (blue).
Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-MAP2 antibody (17490-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, orange), anti-GFAP antibody (16825-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta), and CoraLite®488-conjugated TDP-43 antibody (CL488-10782, green).
Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN antibody (66836-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta), anti-TUBB3 antibody (66375-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, orange), and CoraLite®488-conjugated GFAP antibody (CL488-60190, green).
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP43 antibody (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 antibody (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red), and anti-Lamin B1 antibody (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 antibody (66095-1-Ig) labeled with FlexAble CoraLite® 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, red), and anti-GORASP2 antibody (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HSP60 antibody (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, yellow), anti-GORASP2 antibody (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan), and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-COXIV antibody (11242-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, yellow), anti-GM130 antibody (11308-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta), and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM20 antibody (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, yellow) and anti-Lamin B1 antibody (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD45 antibody (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, cyan) or with isotype control antibody labeled with FlexAble CoraLite® 488 Kit (KFA001, red).
Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® 488 Kit (KFA021).
Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were stained with 0.5 µg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, cyan) or with isotype control antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red).
WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH antibody (10494-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-NUDT21 antibody (66335-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).
WB of HEK293 cell lysates: siRNA transfected HEK293 cell lysates were detected with anti-GNA13 antibody (67188-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022,green) and anti-PCNA antibody (60097-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).
WB of A431 cell lysates: siRNA transfected A431 cell lysates were detected with anti-P53 antibody (80077-1-RR) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-GAPDH antibody (10494-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red).
WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH antibody (10494-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-NUDT21 antibody (66335-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).
WB of HEK293 cell lysates: HEK293 cell lysates were detected with anti-GAPDH antibody (10494-1-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green) and anti-NUDT21 antibody (66335-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, red).
WB of HEK293 cell lysates: HEK293 cell lysates were detected with anti-GAPDH antibody (10494-1-AP) labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, red) and anti-NUDT21 antibody (66335-1-Ig) labeled with FlexAble CoraLite® 488 Kit (KFA021, green).
WB of A431 cell lysates: siRNA transfected A431 cell lysates were detected with anti-P53 antibody (80077-1-RR) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-PCNA antibody (60097-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).
WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 antibody (29824-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-PCNA antibody (60097-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).
FlexAble抗体标记试剂盒是通过高亲和力的FlexLinker®以非共价结合的形式标记抗体。FlexLinker®能在几分钟之内与抗体紧密结合，并且一旦结合就不会轻易从抗体上释放。标记体系中过量的、未结合抗体的FlexLinker®可通过加入猝灭剂FlexQuencher将其中和掉。
经充分测试，FlexAble标记抗体对抗体与靶标的结合没有影响，同时也不会造成抗体损失。
FlexAble抗体标记试剂盒可以与任何供应商的一抗兼容，不论抗体供应商品牌、抗体浓度、以及抗体缓冲液体系。高浓度抗体标记无需稀释，低至0.06mg/mL浓度的抗体也能被有效标记。
FlexAble抗体标记试剂盒适用于任何抗体制剂体系，无需在标记前纯化或者更换缓冲液体系，不会造成抗体的损失，也不受抗体浓度、稳定剂（甘油、BSA等）、含胺缓冲液（Tris等）、防腐剂（叠氮化钠等）等成分的干扰。
Anti-CD3 antibody, previously stored in the indicated concentration of glycerol, was efficiently labeled with FlexAble Antibody Labeling Kit regardless of the glycerol concentration during labeling. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023) . Cells were gated for live singlet lymphocytes.
FlexAble标记抗体仅需一把移液器，两个步骤，10分钟，即可轻松完成抗体标记。
第一步，将一抗、FlexLinker和FlexBuffer混合在一起，孵育5分钟；第二步，加入FlexQuencher以中和未结合的FlexLinker，并再次孵育5分钟。标记完成，管中的即为标记好的抗体，可以直接用于下一步的实验。
FlexAble抗体标记试剂盒可以简化免疫荧光（IF）、蛋白印迹（WB）和流式细胞术（FC）中的多重染色流程，通过在实验前10分钟内完成所有抗体的标记工作，然后同时用所有标记的抗体进行多重染色，简化实验流程，节省孵育时间。
1.使用不同种类的一抗，可以同时在一个管子中用不同的FlexAble抗体标记试剂盒对不同的一抗进行标记。
Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN antibody (66836-1-Ig) labeled with FlexAble CoraLite Plus 647 Kit (KFA023, magenta), anti-TUBB3 antibody 66375-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022, orange), and CoraLite®488-conjugated GFAP antibody (CL488-60190, green).
2.使用相同种类的一抗，可以在不同管子中，使用不同的FlexAble抗体标记试剂盒对不同靶标一抗进行标记，然后将标记好的多个靶标一抗进行混合使用。
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP43 antibody (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 antibody (11802-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, red), and anti-Lamin B1 antibody (12987-1-AP) labeled with FlexAble CoraLite Plus 647 Kit (KFA003, cyan). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
3.FlexAble抗体标记试剂盒标记的抗体，不仅能单独使用，也能配合其他商业化的直标抗体或者其他商业化的一抗二抗（间标）联合使用。
CoraLite Plus光谱
CoraLite®和CoraLite Plus染料是来自Proteintech的明亮荧光染料，具有很高的光稳定性和极小的荧光渗透。我们的FlexAble试剂盒与多种CoraLite®和CoraLite Plus染料结合，在多重标记实验中提供更大的灵活性。
CoraLite®和CoraLite Plus染料的亮度与Alexa Fluor®染料相当。由于荧光光谱重叠极小，每个荧光团可以同时用于共定位研究。FlexAble的CoraLite系列使免疫荧光、蛋白质印迹和流式细胞术的多重检测更快速、更简单。
Flexable 抗体标记试剂盒列表
| 偶联荧光染料 | 规格 | 适配抗体类型 |
|---|---|---|
| CoraLite® Plus 405 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1 |
| FITC Plus | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1 |
| CoraLite® Plus 488 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a NEW |
| CoraLite® Plus 555 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a NEW |
| CoraLite® Plus 647 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1, Mouse IgG2a NEW |
| CoraLite® Plus 750 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1 |
| Conjugate | Size | |
|---|---|---|
| CoraLite Plus 405 NEW | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1 |
| CoraLite 488 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1 |
| CoraLite Plus 555 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1 |
| CoraLite Plus 647 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1 |
| CoraLite Plus 750 | 10 rxn, 50 rxn, 200 rxn | Rabbit IgG, Mouse IgG1 |