CoraLite® Plus 488-conjugated GFAP Monoclonal antibody
GFAP Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4B2E10
验证数据展示
经过测试的应用
Positive IF detected in | mouse brain tissue |
Positive FC detected in | Jurkat cells |
This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:200 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 4 publications below |
产品信息
CL488-60190 targets GFAP in IF, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | FC (Intra), IF/ICC |
Cited Applications | IF |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | mouse, rat |
Immunogen | GFAP fusion protein Ag10452 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | glial fibrillary acidic protein |
Synonyms | GFAP |
Calculated Molecular Weight | 432 aa, 50 kDa |
GenBank Accession Number | BC013596 |
Gene Symbol | GFAP |
Gene ID (NCBI) | 2670 |
RRID | AB_2883116 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P14136 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 GFAP antibody CL488-60190 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Neurosci Res Neuronal deficiency of hypoxia-inducible factor 2α increases hypoxic-ischemic brain injury in neonatal mice. | ||
Front Pharmacol Extracellular Vesicle-Mediated Delivery of Ultrasmall Superparamagnetic Iron Oxide Nanoparticles to Mice Brain. | ||
Brain Res Effects of cannabinoid (CBD) on blood brain barrier permeability after brain injury in rats. | ||