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酪酰胺信号放大（TSA）试剂盒

使用CoraLite® Plus-Tyramide试剂及试剂盒，轻松实现多重成像

  • 灵敏度最高可达间接染色方法的100倍
  • 一抗种属不设限
  • 低丰度靶标轻松呈现
  • 适用石蜡切片/冰冻切片/细胞爬片
Animated diagram illustrating the Tyramide Signal Amplification (TSA) process, showing enzyme-mediated signal enhancement for protein detection.

Proteintech 的 CoraLite® Plus-Tyramide 染料是基于 TSA 技术的荧光标记试剂。在HRP酶催化下，酪胺会被氧化成自由基，随后使染料可以与HRP附近蛋白质上的酪氨酸反应形成共价结合。

与间接染色方法相比，由于染料的积累和对靶标的直接标记，TSA 染料能将荧光信号增强百倍。这使得它们非常适用于检测低丰度靶标。

此外，该系统高度适用于组织多重染色分析，因为可以通过循环式的抗体洗脱与再孵育，依次加入多种 TSA 染料。​

Proteintech的TSA试剂盒产品结合了重组二抗（批间稳定）和自研TSA染料（亮度强、光稳定性好、亲水性强而不易产生背景）优势，无需复杂优化即可以轻松得到显著荧光信号。

查看所有TSA产品

灵敏度显著提高，低丰度靶标检测的理想选择

检测示例：A431 cells，IL-17RA 兔多抗 (货号: 32055-1-AP)

With Multi-rAb CoraLite Plus 594 Recombinant Secondary (RGAR004)​

With Multi-rAb Polymer-HRP Recombinant Secondary (RGAR011) and CoraLite® Plus 594 Tyramide Reagent (PR30024)

实现高质量的多重检测

IF staining using PK10034 (Five color TSA Detect Kit for Rabbit/Mouse Primary Antibody)

FFPE-rat brain tissue was stained with Lamin B1 monoclonal antibody (Cat.NO. 66095-1-Ig) at 1:2000 followed by detection with Multi-rAb® Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L). CoraLite®Plus 555-Tyramide Reagent was used for signal development (yellow). HIER was then performed in pH 9.0 Tris-EDTA buffer followed by staining with GFAP monoclonal antibody (60190-1-Ig, 1:20000). CoraLite®Plus 488-Tyramide Reagent were used for signal development (green). Similarly, IBA1 recombinant antibody (81728-1-RR, 1:2000) TDP-43 recombinant antibody (80002-1-RR, 1:3200) were stained subsequently. CoraLite®Plus 594-Tyramide Reagent (red) and CoraLite®Plus 647-Tyramide Reagent (purple) were used for signal development, respectively. Finally, cell nucleus (blue) were stained with DAPI.​​

IF staining using using PK10034 (Five color TSA Detect Kit for Rabbit/Mouse Primary Antibody).

FFPE-human tonsillitis tissue was stained with CD23 monoclonal antibody (60208-2-Ig) at 1:8000 followed by detection with Multi-rAb® Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L). CoraLite®Plus 555-Tyramide Reagent was used for signal development (yellow). HIER was then performed in pH 9.0 Tris-EDTA buffer followed by staining with CD3 monoclonal antibody (60181-1-Ig, 1:10000). CoraLite®Plus 488-Tyramide Reagent were used for signal development (green). Similarly, CD8a monoclonal antibody (66868-1-Ig, 1:20000) and CD138 monoclonal antibody (67155-1-Ig, 1:10000) were stained subsequently. CoraLite®Plus 594-Tyramide Reagent (red) and CoraLite®Plus 647-Tyramide Reagent (purple) were used for signal development respectively. Finally, cell nucleus (blue) were stained with DAPI.​

产品列表

TSA检测试剂盒

该试剂盒为组织多重染色提供了一站式免疫荧光解决方案。 组分：包含3或4种TSA染料、细胞核染料DAPI、重组二抗、阻断剂、封闭/稀释缓冲液及扩增液

产品名称 货号
3标4色抗兔/小鼠通用型TSA检测试剂盒 PK10032
3标4色抗兔TSA检测试剂盒 PK10033
4标5色抗兔/小鼠通用型TSA检测试剂盒 PK10034
4标5色抗兔TSA检测试剂盒 PK10035

CoraLite® Plus- Tyramide试剂

可搭配任何HRP标记的二抗使用，适用于多重免疫荧光、原位杂交等实验

染料 货号
CoraLite® Plus 488-TSA染料 PR30022
CoraLite® Plus 555-TSA染料 PR30023
CoraLite® Plus 594-TSA染料 PR30024
CoraLite® Plus 647-TSA染料 PR30025