-
纯度 > 95%
-
超低内毒素水平 < 1EU/mg
-
不含甘油、蛋白保护剂和防腐剂
-
聚集程度 < 5%
-
0.22 μm滤膜过滤除菌
-
无动物源生产工艺制备
-
大包装规格（mg 到 g 的规格）
-
可定制规格和浓度
-
适用于体内外功能研究，也可以用于FC、ELISA等实验
数据验证
1x10^6 human PBMCs were surface stained with 0.25 ug LE/AF Purified Rabbit Anti-Human CD28 Antibody (Cat. No. BK00001, Clone: 240354D2) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.25 ug LE/AF Purified Mouse Anti-Human CD3 Antibody (Cat. No. BK00002, Clone: OKT3) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 750 Anti-Human CD19 Rabbit Recombinant Antibody (CL750-98560, Clone: 250499A2). Cells were not fixed. Lymphocytes were gated.
Purity of LE/AF Purified Rabbit Anti-Human CD28 Antibody (Cat. No. BK00001, Clone: 240354D2) was determined by SDS-PAGE. The antibody was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.
Purity of LE/AF Purified Mouse Anti-Human CD3 Antibody (Cat. No. BK00002, Clone: OKT3) was determined by SDS-PAGE. The antibody was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.
1x10^6 untreated or anti-CD3 (BK00002, 2 ug/ml) and anti-CD28 (BK00001, 10 ug/ml) treated human PBMCs were surface stained with PE Anti-Human CD4 and 5 ul APC Anti-Human TIM3 Rabbit RecAb (APC-FcA98115, Clone: 241422H1). Cells were not fixed.
Biolayer interferometry (BLl) kinetic assays of BK00002 against Human CD3 were performed. The affinity constant is 2.20 nM.
