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验证数据展示

  • FC experiment of human PBMCs using BK00001

    FC experiment of human PBMCs using BK00001

    1x10^6 human PBMCs were surface stained with 0.25 ug LE/AF Purified Rabbit Anti-Human CD28 Antibody (BK00001, Clone:240354D2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were not fixed. Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using BK00001

    FC experiment of human PBMCs using BK00001

    1x10^6 human PBMCs were surface stained with 0.25 ug LE/AF Purified Rabbit Anti-Human CD28 Antibody (BK00001, Clone:240354D2) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

  • Functional Assay experiment of human PBMCs using BK00001

    Functional Assay experiment of human PBMCs using BK00001

    1x10^6 untreated or anti-CD3 (BK00002, 2 ug/ml) and anti-CD28 (BK00001, 10 ug/ml) treated human PBMCs were surface stained with 5 ul FcZero-rAb® PE Anti-Human CD69 Rabbit Recombinant Antibody (PE-FcA98173, Clone: 241552F1) and 5 ul FcZero-rAb® APC Anti-Human CD25/IL-2RA Rabbit Recombinant Antibody (APC-FcA98469-4, Clone: 253320A1). Cells were not fixed.

  • SEC-HPLC of BK0001

    SEC-HPLC of BK0001

    The purity of Human CD28 Recombinant monoclonal antibody (Cat. No. 98032-1-FG) was greater than 95% as determined by SEC-HPLC.

  • SDS-PAGE of BK0001

    SDS-PAGE of BK0001

    Purity of Human CD28 Recombinant monoclonal antibody (Cat. No. 98032-1-FG) was determined by SDS-PAGE. The antibody was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Affinity of BK0001

    Affinity of BK0001

    Biolayer interferometry (BLl) kinetic assays of 98032-1-FG against Human CD28 were performed. The affinity constant is 2.20 nM.

LE/AF Purified Rabbit Anti-Human CD28 Antibody

Low-endotoxin/Azide-free (LE/AF) Purified CD28 Recombinant Antibody for FC, Functional Assay
Cat No. BK00001

产品说明书

CloneNo. 240354D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, Functional Assay

别名

CD28 molecule, T-cell-specific surface glycoprotein CD28, Tp44

缓冲液配方:  Sterile PBS
Sterile PBS
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive Functional Assay detected inhuman PBMCs
Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
FUNCTIONAL ASSAYFUNCTIONAL ASSAY : Assay Dependent
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

BK00001 targets CD28 in FC, Functional Assay applications and shows reactivity with human samples.

经测试应用 FC, Functional Assay Application Description
经测试反应性 human
免疫原

CatNo: Eg0889

Product name: Recombinant Human CD28 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 19-152 aa of BC093698

Sequence: NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD28 molecule
别名 CD28 molecule, T-cell-specific surface glycoprotein CD28, Tp44
计算分子量 220 aa, 25 kDa
GenBank蛋白编号BC093698
基因名称 CD28
Gene ID (NCBI) 940
ENSEMBL Gene IDENSG00000178562
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
纯度≥98% (HPLC), ≥98% (SDS-PAGE)
无菌性0.22 µm filtration
UNIPROT IDP10747
储存缓冲液 Sterile PBS, pH 7.4.
储存条件Store at 2 - 8°C. Stable for one year after shipment.
内毒素<0.1 EU/mg

背景介绍

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

实验方案

Product Specific Protocols
FC protocol for CD28 antibody BK00001Download protocol
Standard Protocols
Click here to view our Standard Protocols
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