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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Compatible Species
0
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验证数据展示

  • FC experiment of human PBMCs using 98136-1-RR

    FC experiment of human PBMCs using 98136-1-RR

    1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed.

  • IP experiment of HepG2 using 98136-1-RR

    IP experiment of HepG2 using 98136-1-RR

    IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug.

  • IF Staining of HeLa using 98136-1-RR

    IF Staining of HeLa using 98136-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control.

  • FC experiment of SH-SY5Y using 98136-1-RR

    FC experiment of SH-SY5Y using 98136-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis using 98136-1-RR

    WB analysis using 98136-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 98136-1-RR

    IHC staining of mouse colon using 98136-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • ChIP experiment of HeLa using 98136-1-RR

    ChIP experiment of HeLa using 98136-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2A (Lys9) (83041-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR
Cat No. 98136-1-RR

产品说明书

CloneNo. 240953C9
引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR

别名

Rabbit IgG, Rabbit IgG Control, Rabbit IgG isotype, Rabbit IgG Isotype Control

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in
Positive IP detected in
Positive IHC detected in
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Positive FC (Intra) detected in
Positive FC detected in
Positive ChIP-qPCR detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension
Flow Cytometry (FC)FC : 0.2 ug per 10^6 cells in 100 μl suspension
CHIP-QPCRCHIP-QPCR : 1:10-1:100
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 3 publications below

产品信息

98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR applications and shows reactivity with n/a samples.

经测试应用 WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR Application Description
文献引用应用IP
经测试反应性 n/a
免疫原

Small molecule

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IgG Isotype Control
别名 Rabbit IgG, Rabbit IgG Control, Rabbit IgG isotype, Rabbit IgG Isotype Control
基因名称
Gene ID (NCBI)
RRIDAB_3672282
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

实验方案

Product Specific Protocols
IF protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IHC protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IP protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
WB protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IP

Biochim Biophys Acta Mol Basis Dis

Ischemia-reperfusion injury induces ZBP1-dependent PANoptosis in endothelial cells

Authors - Yue Cui
View Article
IP

Autophagy

ATG9 inhibits rickettsia binding to the Host cell surface by blocking the rOmpB-XRCC6/KU70 interaction

Authors - Chen Chen
View Article
IP

Acta Pharmacol Sin

Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway

Authors - Hai-Qi Wu
View Article
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