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验证数据展示

  • FC experiment of human PBMCs using 98136-1-RR (same clone as 98136-1-PBS)

    FC experiment of human PBMCs using 98136-1-RR (same clone as 98136-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 98136-1-RR (same clone as 98136-1-PBS)

    IP experiment of HepG2 using 98136-1-RR (same clone as 98136-1-PBS)

    IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

    IF Staining of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 98136-1-RR (same clone as 98136-1-PBS)

    FC experiment of SH-SY5Y using 98136-1-RR (same clone as 98136-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • WB analysis using 98136-1-RR (same clone as 98136-1-PBS)

    WB analysis using 98136-1-RR (same clone as 98136-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 98136-1-RR (same clone as 98136-1-PBS)

    IHC staining of mouse colon using 98136-1-RR (same clone as 98136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • ChIP experiment of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

    ChIP experiment of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2A (Lys9) (83041-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

Rabbit IgG Isotype Control Recombinant Antibody, PBS Only

IgG Isotype Control Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR
Cat No. 98136-1-PBS

产品说明书

CloneNo. 240953C9

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR

别名

Rabbit IgG, Rabbit IgG Control, Rabbit IgG isotype, Rabbit IgG Isotype Control

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98136-1-PBS targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR applications and shows reactivity with n/a samples.

经测试应用 WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR Application Description
经测试反应性 n/a
免疫原

Small molecule

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IgG Isotype Control
别名 Rabbit IgG, Rabbit IgG Control, Rabbit IgG isotype, Rabbit IgG Isotype Control
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

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