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CCDC111/PRIMPOL Polyclonal antibody

CCDC111/PRIMPOL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29824-1-AP

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Synonyms

CCDC111, FLJ33167, hPrimpol1, PRIMPOL



经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

产品信息

29824-1-AP targets CCDC111/PRIMPOL in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC, WB, ELISA
Tested Reactivity Human
Immunogen CCDC111/PRIMPOL fusion protein Ag31478 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 111
Synonyms CCDC111, FLJ33167, hPrimpol1, PRIMPOL
Observed molecular weight64 kDa
GenBank accession numberBC064600
Gene symbol CCDC111
UniprotQ96LW4
Gene ID (NCBI) 201973
RRIDAB_2918349
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).

实验方案

Product Specific Protocols
WB protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
IHC protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
IF protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols