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GAPDH Polyclonal antibody

GAPDH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, arabidopsis , corn , cabbage, rice and More (8)

Applications

FC, IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10494-1-AP

Print datasheet

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

验证数据展示

  • WB analysis using 10494-1-AP

    WB analysis using 10494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10494-1-AP

    WB analysis using 10494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10494-1-AP (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 10494-1-AP

    IP experiment of A549 using 10494-1-AP

    IP Result of anti-GAPDH (IP:10494-1-AP, 3ug; Detection:10494-1-AP 1:3000) with A549 cells lysate 3500ug.

  • IHC staining of human breast cancer using 10494-1-AP

    IHC staining of human breast cancer using 10494-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GAPDH antibody included
  • IHC staining of human breast cancer using 10494-1-AP

    IHC staining of human breast cancer using 10494-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GAPDH antibody included
  • IF Staining of HeLa using 10494-1-AP

    IF Staining of HeLa using 10494-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH antibody (10494-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • FC experiment of HEK-293 using 10494-1-AP

    FC experiment of HEK-293 using 10494-1-AP

    1X10^6 HEK-293 cells were stained with .2ug GAPDH antibody (10494-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.

经过测试的应用

Positive WB detected inhuman placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

产品信息

10494-1-AP targets GAPDH in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn , cabbage, rice samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications CoIP, ELISA, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat, pig, arabidopsis , corn , cabbage, rice
Cited Reactivityhuman, rat, goat, treeshrew, Chicken, Yeast, leech, Arabidopsis, zebra finches, sheep
Immunogen GAPDH fusion protein Ag0766 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, GAPDH, OK/SW cl.12
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
UniprotP04406
Gene ID (NCBI) 2597
RRIDAB_2263076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

实验方案

Product Specific Protocols
WB protocol for GAPDH antibody 10494-1-APDownload protocol
IHC protocol for GAPDH antibody 10494-1-APDownload protocol
IF protocol for GAPDH antibody 10494-1-APDownload protocol
IP protocol for GAPDH antibody 10494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
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mouseWB

Nature

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humanWB

Nature

Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts tropism and fusogenicity.

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human,mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
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humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
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humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
View Article