验证数据展示
经过测试的应用
| Positive IF-P detected in | human kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-18150 targets Podocalyxin in IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human |
| 免疫原 | Podocalyxin fusion protein Ag12844 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | podocalyxin-like |
| 别名 | PODXL, PCLP 1, PCLP, PC, Gp200 |
| 计算分子量 | 526 aa, 55 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC093730 |
| 基因名称 | Podocalyxin |
| Gene ID (NCBI) | 5420 |
| RRID | AB_2919156 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00592 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It is Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in many cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Podocalyxin antibody CL488-18150 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |