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GOLGA2/GM130 Polyclonal antibody

GOLGA2/GM130 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11308-1-AP

Print datasheet

Synonyms

GM130, GOLGA2, Golgin 95, GM130;GOLGA2, GM130 autoantigen



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, human spleen tissue, HEK-293 cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MDCK cells, HEK-293 cells, HeLa cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, canine samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, canine
Cited Reactivityhuman, monkey, hamster
Immunogen GOLGA2/GM130 fusion protein Ag1848 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 2
Synonyms GM130, GOLGA2, Golgin 95, GM130;GOLGA2, GM130 autoantigen
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC014188
Gene Symbol GM130
Gene ID (NCBI) 2801
RRIDAB_2115327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

实验方案

Product Specific Protocols
WB protocol for GOLGA2/GM130 antibody 11308-1-APDownload protocol
IHC protocol for GOLGA2/GM130 antibody 11308-1-APDownload protocol
IF protocol for GOLGA2/GM130 antibody 11308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng

Immunity

The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity

Authors - Tian Chen
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
humanIF

Adv Mater

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Authors - Zhuo Li
WB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
humanWB,IF

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang