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验证数据展示

  • IF Staining of HepG2 using CL647-11308

    IF Staining of HepG2 using CL647-11308

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 GOLGA2/GM130 antibody (CL647-11308) at dilution of 1:200.

  • FC experiment of HeLa using CL647-11308

    FC experiment of HeLa using CL647-11308

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GOLGA2/GM130 (CL647-11308) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated GOLGA2/GM130 Polyclonal antibody

GOLGA2/GM130 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-11308

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

IF/ICC, FC (Intra)

别名

GM130, GOLGA2, Golgin 95, GM130;GOLGA2, GM130 autoantigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOLGA2/GM130 Ready-To-Use IHC Kit. GOLGA2/GM130 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11308-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-11308 targets GOLGA2/GM130 in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, canine
免疫原

CatNo: Ag1848

Product name: Recombinant human GM130;GOLGA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 361-678 aa of BC014188

Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 2
别名 GM130, GOLGA2, Golgin 95, GM130;GOLGA2, GM130 autoantigen
计算分子量 111 kDa
观测分子量 130 kDa
GenBank蛋白编号BC014188
基因名称 GM130
Gene ID (NCBI) 2801
RRIDAB_2934854
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08379
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein, one component of the detergent and salt-resistant Golgi matrix. It is a peripheral membrane protein highly bound to the Golgi membrane and localized mainly at the cytoplasmic face of the cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in regulating ER-to-Golgi transport and maintaining the Golgi structure. Emerging evidence suggests that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher-order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 GOLGA2/GM130 antibody CL647-11308Download protocol
FC protocol for CL Plus 647 GOLGA2/GM130 antibody CL647-11308Download protocol
Standard Protocols
Click here to view our Standard Protocols