Anti-Human CD45 (HI30)

CD45 Monoclonal Antibody for IF/ICC, FC
Cat No. 65109-1-Ig

产品说明书

CloneNo. HI30

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

IF/ICC, FC and More (1)

EC:3.1.3.48, GP180, HI30, L CA, LCA

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65109-1-Ig targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
文献引用应用IF, FC
经测试反应性 human
文献引用反应性human
免疫原 Human peripheral blood leucocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 EC:3.1.3.48, GP180, HI30, L CA, LCA
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2918407
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP08575
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CD45 antibody 65109-1-IgDownload protocol
FC protocol for CD45 antibody 65109-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

Front Cell Dev Biol

Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.

Authors - Xun Xi
humanIF

Cell Discov

Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics

Authors - Min Tang
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