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Lamin B1 Monoclonal antibody
Lamin B1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat No : 66095-1-Ig
|Positive WB detected in||NCI-H1299 cells, multi-cells/tissue, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human pancreas cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, mouse eye tissue, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66095-1-Ig is Lamin B1 Fusion Protein expressed in E. coli.
|Tested Applications||FC, IF, IHC, IP, WB, ELISA|
|Cited Applications||IF, IHC, IP, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Rabbit, zebrafish|
|Immunogen||Lamin B1 fusion protein Ag20522|
|Host / Isotype||Mouse / IgG1|
|Full Name||lamin B1|
|Synonyms||ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66-70 kDa|
|GenBank accession number||BC012295|
|Gene ID (NCBI)||4001|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.
|Product Specific Protocols|
|WB protocol for Lamin B1 antibody 66095-1-Ig||Download protocol|
|IHC protocol for Lamin B1 antibody 66095-1-Ig||Download protocol|
|IF protocol for Lamin B1 antibody 66095-1-Ig||Download protocol|
|IP protocol for Lamin B1 antibody 66095-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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