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Lamin B1 Monoclonal antibody

Lamin B1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C10G12

Cat No : 66095-1-Ig

Print datasheet

Synonyms

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1



经过测试的应用

Positive WB detected inNCI-H1299 cells, multi-cells/tissue, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse eye tissue, HeLa cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 66095-1-Ig is Lamin B1 Fusion Protein expressed in E. coli.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Rabbit, zebrafish
Immunogen Lamin B1 fusion protein Ag20522 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lamin B1
Synonyms ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC012295
Gene symbol LMNB1
UniprotP20700
Gene ID (NCBI) 4001
RRIDAB_11232208
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

实验方案

Product Specific Protocols
WB protocol for Lamin B1 antibody 66095-1-IgDownload protocol
IHC protocol for Lamin B1 antibody 66095-1-IgDownload protocol
IF protocol for Lamin B1 antibody 66095-1-IgDownload protocol
IP protocol for Lamin B1 antibody 66095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Cell Biol

Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation.

Authors - Subhash B Arya

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai
humanWB

Cell Host Microbe

Structural Basis for a Species-Specific Determinant of an SIV Vif Protein toward Hominid APOBEC3G Antagonism.

Authors - Jennifer M Binning
human,mouseWB,IF

Cell Host Microbe

The Nuclear Matrix Protein SAFA Surveils Viral RNA and Facilitates Immunity by Activating Antiviral Enhancers and Super-enhancers.

Authors - Lili Cao
humanWB

Mol Cell

Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.

Authors - Wenqi Xu
humanWB

Hepatology

Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.

Authors - Meng Lu