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- KD/KO Validated
Lamin B1 Monoclonal antibody
Lamin B1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (2)
Applications
FC, IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
237
CloneNo.
3C10G12
验证数据展示
经过测试的应用
| Positive WB detected in | NCI-H1299 cells, multi-cells/tissue, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, mouse eye tissue, HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:250-1:1000 |
| Sample-dependent, check data in validation data gallery | |
产品信息
The immunogen of 66095-1-Ig is Lamin B1 Fusion Protein expressed in E. coli.
| Tested Applications | FC, IF, IHC, IP, WB, ELISA |
| Cited Applications | IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, Rabbit, zebrafish |
| Immunogen | Lamin B1 fusion protein Ag20522 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lamin B1 |
| Synonyms | ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1 |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66-70 kDa |
| GenBank accession number | BC012295 |
| Gene symbol | LMNB1 |
| Uniprot | P20700 |
| Gene ID (NCBI) | 4001 |
| RRID | AB_11232208 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
| IHC protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
| IF protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
| IP protocol for Lamin B1 antibody 66095-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation. | ||
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Cell Host Microbe Structural Basis for a Species-Specific Determinant of an SIV Vif Protein toward Hominid APOBEC3G Antagonism. | ||
Cell Host Microbe The Nuclear Matrix Protein SAFA Surveils Viral RNA and Facilitates Immunity by Activating Antiviral Enhancers and Super-enhancers. | ||
Mol Cell Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis. | ||
Hepatology Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress. |
