验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, fetal human brain tissue, HEK-293 cells, PC-12 cells, Neuro-2a cells, Pig brain, Rabbit brain, Rat brain Mouse brain, Chicken brain |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human cerebellum tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue, rat brain tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, Retinal organoids, mouse brain tissue, human neuron |
| Positive IF-Fro detected in | rat brain tissue, rat cerebellum tissue |
| Positive IF/ICC detected in | iPS cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66375-1-Ig targets TUBB3-specific/TUJ1 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit, chicken |
| 文献引用反应性 | human, mouse, rat, rabbit, chicken |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tubulin, beta 3 |
| 别名 | TUBB3, TUBB3-specific, TUJ1, beta tubulin, Tubulin beta 3 chain |
| 计算分子量 | 55 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | NM_001197181 |
| 基因名称 | TUBB3 |
| Gene ID (NCBI) | 10381 |
| RRID | AB_2814998 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13509 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for TUBB3-specific/TUJ1 antibody 66375-1-Ig | Download protocol |
| IF protocol for TUBB3-specific/TUJ1 antibody 66375-1-Ig | Download protocol |
| IHC protocol for TUBB3-specific/TUJ1 antibody 66375-1-Ig | Download protocol |
| IP protocol for TUBB3-specific/TUJ1 antibody 66375-1-Ig | Download protocol |
| WB protocol for TUBB3-specific/TUJ1 antibody 66375-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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