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Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 10712-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (5)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
规格价格库存


经过测试的应用

Positive WB detected inA431 cells, mouse placenta tissue, mouse brain tissue, A549 cells, HepG2 cells, HT-29 cells, MCF-7 cells, T-47D cells, SK-BR-3 cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, human colon cancer tissue, human lung cancer tissue, human oesophagus cancer tissue, human pancreas tissue, human skin tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse colon tissue, mouse pancreas tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inRetinal organoids, mouse pancreas tissue, mouse small intestine tissue
Positive IF/ICC detected inHaCaT cells, HeLa cells, HepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)-PIF-P : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10712-1-AP targets Cytokeratin 19 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, pig, canine, goat, sheep
免疫原

CatNo: Ag1085

Product name: Recombinant human CK19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-400 aa of BC007628

Sequence: EKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 19
别名 CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin
计算分子量 40 kDa
观测分子量 44-50 kDa
GenBank蛋白编号BC007628
基因名称 Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2133325
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08727
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.

实验方案

Product Specific Protocols
FC protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
WB protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC,IF

Nature

Evolutionary fingerprints of epithelial-to-mesenchymal transition

Authors - Luigi Perelli

Exploration (Beijing)

Peptide RL-QN15 Regulates Functions of Epidermal Stem Cells to Accelerate Skin Wound Regeneration via the FZD8/β-Catenin Axis.

Authors - Yuansheng Li
mouseIF

Gut

ISG15 deficiency in hepatic stellate cells promotes TGFβ2-induced liver fibrosis by counteracting CREB1 ISGylation

Authors - Yue Yuan
humanIF

Gut

Novel microenvironment-based classification of intrahepatic cholangiocarcinoma with therapeutic implications

Authors - Miguel A Martin-Serrano

Cell Host Microbe

Zika Virus Targets Different Primary Human Placental Cells, Suggesting Two Routes for Vertical Transmission.

Authors - Takako Tabata

Drug Resist Updat

NDRG1 overcomes resistance to immunotherapy of pancreatic ductal adenocarcinoma through inhibiting ATG9A-dependent degradation of MHC-1

Authors - Zhiheng Zhang
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