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验证数据展示

  • IF Staining of HeLa using CL555-10712

    IF Staining of HeLa using CL555-10712

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 555 Cytokeratin 19 antibody (CL555-10712) at dilution of 1:200.

  • FC experiment of A431 using CL555-10712

    FC experiment of A431 using CL555-10712

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 555 Anti-Human Cytokeratin 19 (CL555-10712) and HRP-conjugated polymer at dilution 1:1000 (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 555-conjugated Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-10712

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

CK19, CK 19, KRT19, K19, Cytokeratin-19

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10712-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-10712 targets Cytokeratin 19 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Cytokeratin 19 fusion protein Ag1085 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 19
别名 CK19, CK 19, KRT19, K19, Cytokeratin-19
计算分子量 40 kDa
观测分子量 44-50 kDa
GenBank蛋白编号BC007628
基因名称 Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_3084522
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08727
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

实验方案

Product Specific Protocols
IF protocol for CL Plus 555 Cytokeratin 19 antibody CL555-10712Download protocol
FC protocol for CL Plus 555 Cytokeratin 19 antibody CL555-10712Download protocol
Standard Protocols
Click here to view our Standard Protocols