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TUBB3-specific Monoclonal antibody, PBS Only

TUBB3-specific Monoclonal Antibody for WB,IHC,IF,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken, pig

Applications

WB,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1F8G10

Cat No : 66375-1-PBS

Print datasheet

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1, tuj1 antibody

验证数据展示

  • IF Staining of iPS cells using 66375-1-Ig (same clone as 66375-1-PBS)

    IF Staining of iPS cells using 66375-1-Ig (same clone as 66375-1-PBS)

    Immunofluorescent staining of TUBB3 (66375-1-lg, 1:250) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (Green: TUBB3; Blue: DAPI). Provided by BioTalentum Ltd., Hungary. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

  • IF Staining of Retinal organoids using 66375-1-Ig (same clone as 66375-1-PBS)

    IF Staining of Retinal organoids using 66375-1-Ig (same clone as 66375-1-PBS)

    Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

  • IF Staining of human neuron using 66375-1-Ig (same clone as 66375-1-PBS)

    IF Staining of human neuron using 66375-1-Ig (same clone as 66375-1-PBS)

    Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

  • IF Staining of Retinal organoids using 66375-1-Ig (same clone as 66375-1-PBS)

    IF Staining of Retinal organoids using 66375-1-Ig (same clone as 66375-1-PBS)

    Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 66375-1-Ig (same clone as 66375-1-PBS)

    IF Staining of rat brain using 66375-1-Ig (same clone as 66375-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66375-1-Ig (TUBB3-specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 26975-1-AP (NeuN antibody, red). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

  • WB analysis using 66375-1-Ig (same clone as 66375-1-PBS)

    WB analysis using 66375-1-Ig (same clone as 66375-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:49000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66375-1-Ig (same clone as 66375-1-PBS)

    FC experiment of SH-SY5Y using 66375-1-Ig (same clone as 66375-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human TUBB3-specific (66375-1-Ig, Clone:1F8G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66375-1-Ig (same clone as 66375-1-PBS)

    IHC staining of mouse brain using 66375-1-Ig (same clone as 66375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

    IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

    IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • WB analysis of fetal human brain using 66375-1-Ig (same clone as 66375-1-PBS)

    WB analysis of fetal human brain using 66375-1-Ig (same clone as 66375-1-PBS)

    Western blot analysis of TUBB3 in various tissues and cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

  • IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

    IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

    IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.

产品信息

66375-1-PBS targets TUBB3-specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken, pig samples.

Tested Applications WB,IHC,IF,FC,ELISA
Tested Reactivity human, mouse, rat, rabbit, chicken, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1, tuj1 antibody
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberNM_001197181
Gene Symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.