Lamin B1 Polyclonal antibody
Lamin B1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
FC, IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
769
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse kidney tissue, mouse pancreas tissue, SH-SY5Y cells, mouse lung tissue, mouse eye tissue, Jurkat cells, RAW 264.7 cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, human skin cancer tissue, NIH/3T3 cells |
| Positive FC detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
产品信息
12987-1-AP targets Lamin B1 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF, IHC, IP, WB, ELISA |
| Cited Applications | ChIP, FC, IF, IHC, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, Bombyx mori, canine, chicken, hamster, pig |
| Immunogen | Lamin B1 fusion protein Ag3631 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lamin B1 |
| Synonyms | ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1 |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66-70 kDa |
| GenBank accession number | BC012295 |
| Gene symbol | LMNB1 |
| Uniprot | P20700 |
| Gene ID (NCBI) | 4001 |
| RRID | AB_2136290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Lamin B1 antibody 12987-1-AP | Download protocol |
| IHC protocol for Lamin B1 antibody 12987-1-AP | Download protocol |
| IF protocol for Lamin B1 antibody 12987-1-AP | Download protocol |
| IP protocol for Lamin B1 antibody 12987-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases | ||
Nature Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1. | ||
Cancer Discov Posttranslational regulation of the exon skipping machinery controls aberrant splicing in leukemia. | ||
Immunity Targeting 7-Dehydrocholesterol Reductase Integrates Cholesterol Metabolism and IRF3 Activation to Eliminate Infection. |
