Lamin B1 Polyclonal antibody
Lamin B1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
FC, IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
903
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse pancreas tissue, SH-SY5Y cells, mouse kidney tissue, mouse lung tissue, mouse eye tissue, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse heart tissue, human kidney tissue, mouse kidney tissue, human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, human skin cancer tissue, NIH/3T3 cells |
Positive FC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
产品信息
12987-1-AP targets Lamin B1 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | ChIP, FC, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, hamster, pig, Bombyx mori, canine |
Immunogen | Lamin B1 fusion protein Ag3631 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lamin B1 |
Synonyms | ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1 |
Calculated molecular weight | 66 kDa |
Observed molecular weight | 66-70 kDa |
GenBank accession number | BC012295 |
Gene symbol | LMNB1 |
Uniprot | P20700 |
Gene ID (NCBI) | 4001 |
RRID | AB_2136290 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Lamin B1 antibody 12987-1-AP | Download protocol |
IHC protocol for Lamin B1 antibody 12987-1-AP | Download protocol |
IF protocol for Lamin B1 antibody 12987-1-AP | Download protocol |
IP protocol for Lamin B1 antibody 12987-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Biotechnol Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases | ||
Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies | ||
Nature Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1. | ||
Cell Host Microbe Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice |