Cerebral Organoids

HumanKine® Growth Factors are the perfect tool for your cerebral organoid research


Cerebral organoids are a powerful tool to study neurodevelopment and implementing drug screening for neurological disorders. These structures are generated from induced or human pluripotent stem cells (iPSCs, hPSCs) grown in specialized media with growth factors.

HumanKine® recombinant cytokines and growth factors are authentic human proteins that are HEK293 cell expressed, and demonstrate greater activity than E. coli-expressed proteins. They are also tag and endotoxin-free.

 

Don't risk your organoid cultures with poor quality proteins: Choose HumanKine® growth factors
Target
Purity
Activity (EC50)
Source
Model Organoid
GMP-grade?

BMP4

>95%

1.5-9 ng/mL

HEK293

Hippocampal-Choroid Plexus

Yes

EGF

>95%

Active

HEK293

Cortical

 

FGFb

>95%

0.05-0.4 ng/mL

HEK293

Cortical, Cerebellar

Yes

FGF8

>95%

≤ 10 ng/mL

HEK293

Midbrain

 

GDNF

>95%

≤ 10 ng/mL

HEK293

Midbrain, Hypothalamus, Forebrain

 

SHH

>90%

≤ 350 ng/mL

HEK293

Midbrain

 

TGF

>95%

≤ 0.5 ng/mL

HEK293

Midbrain, Hypothalamus, Forebrain

Yes

WNT3A

>90%

2-17 ng/mL

HEK293

Midbrain, Hypothalamus, Forebrain

Yes

 

HumanKine® FGFb-TS protein

Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line graph.

Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line.

 

Know your organoids with neuronal and progenitor markers
Pax6 polyclonal antibody
SOX2 polyclonal antibody
TUJ1 monoclonal antibody
immunofluorescence (IF) staining of iPS cells using Proteintech PAX6 antibody (12323-1-AP) immunofluorescence (IF) staining of iPS cells using Proteintech SOX2 antibody (11064-1-AP) immunofluorescence (IF) staining of iPS cells using Proteintech TUBB3 antibody (66375-1-Ig)

PAX6 (12323-1-AP, green) neuronal precursor cells (NPCs). Blue: DAPI. Provided by Bio Talentum Ltd., Hungary.

SOX2 (11064-1-AP, green)-positive cells in mouse brain tissue. Blue: DAPI

TUJ1/TUBB3 (66375-1-lg, green) hiPSC derived neuronal cultures (35 days old). Blue: DAPI.
Provided by BioTalentum Ltd., Hungary.

 

Antibodies for brain organoid characterization
Target Reactivity Host Application Cell/Tissue Type
CTIP2/BCL11B Human, Mouse Rabbit WB, IP. IF Deep-layer cortical neurons
DCX Human, Mouse, Rat Rabbit WB, IHC, IF, ELISA Developing neurons
DLX2 Human, Mouse Rabbit WB, IHC, IF, ELISA NSCs, forebrain
EMX1 Human Mouse, Rat Rabbit WB, ELISA Forebrain NSCs
EOMES/TBR2 Human, Mouse, Rat Rabbit WB, ELISA Intermediate progenitors
FOXA2 Human, Mouse Rabbit WB, IP, IHC, IF, ChIP, ELISA Midbrain dopaminergic neurons
FOXG1 Human, Mouse, Rat Rabbit WB, ELISA Forebrain cells
FZD9 Human, Mouse, Rat Rabbit WB, IHC, ELISA Cortical precursors, hippocampal neurons
GAD1 Human, Mouse, Rat Rabbit WB, IP, IHC, IF, ELISA Ventral forebrain
KIRREL2 Human, Mouse, Rat Rabbit WB, ELISA Cerebellar progenitors
LHX Human Rabbit WB, ELISA Ventral forebrain
NCAD Human, Mouse Rat, Bovine, Canine, Gecko, Horse Rabbit WB, IP, IHC, IF, FC, CoIP, ELISA NSCs, migrating neurons, neural plate
NELL2 Human Rabbit WB, IHC, IF, ELISA NSCs, neurons
Nestin Human, Mouse, Rat Rabbit WB, IHC, IF, ELISA NSCs, progenitors GABAergic interneurons, oligodendrocytes
NKX2.1 Human, Mouse, Rat Rabbit WB, ELISA ventral forebrain
OTX2 Human, Mouse, Rat, Chicken Rabbit WB, IP, IHC, IF, FC, ChIP, ELISA Midbrain cells
PAX6 Human, Mouse, Rat Rabbit WB, IHC, IF, FC, ELISA Radial glia/NSCs
PROX1 Human, Mouse, Rat Rabbit WB, IF, FC, ChIP, ELISA Hippocampal neurons
Reelin Human, Mouse Rabbit IHC, IF, ELISA Pre-plate/Cajal-retzius cells
SATB2 Human, Mouse Mouse WB, IHC, ELISA Cortical neurons across corpus callosum
SOX2 Human, Mouse Rat, Zebrafish Rabbit WB, IHC, IF, FC, ELISA Radial glia/NSCs
TBR1 Human, Mouse, Rat Rabbit WB, IP, IHC, IF, ELISA Pre-plate/Cajal-retzius cells
TH Human, Mouse, Rat, Canine, Gerbil, Zebrafish Rabbit WB, IP, IHC, IF, ELISA Midbrain NSCs
TTR Human, Mouse Rabbit WB, IHC, IF, ELISA Choroid plexus
TUJ1/TUBB3 Human, Mouse, Rat Mouse WB, IHC, FC, ELISA Neurons
VGAT C. elegans, Human, Mouse, Rat Rabbit WB, IP, IHC, IF, ELISA GABAergic and glycinergic neurons
Vimentin Human, Mouse, Rat, Bovine, Deer,Hamster, Pig Rabbit WB, IHC, IF, FC, ELISA Radial glia/NSCs