TBR1 Polyclonal antibody
TBR1 Polyclonal Antibody for IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy TBR1 Ready-To-Use IHC Kit. TBR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 20932-1-AP is TBR1 Fusion Protein expressed in E. coli.
| Tested Applications | IF-P, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC, FC (Intra) |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | TBR1 fusion protein Ag14935 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | T-box, brain, 1 |
| Synonyms | T box brain protein 1, T box, brain, 1, T brain 1, TBR 1, TBR1, TES 56 |
| Calculated Molecular Weight | 682 aa, 74 kDa |
| Observed Molecular Weight | 74 kDa |
| GenBank Accession Number | BC104844 |
| Gene Symbol | TBR1 |
| Gene ID (NCBI) | 10716 |
| RRID | AB_10695502 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16650 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBR1, also named as T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBR1 antibody 20932-1-AP | Download protocol |
| IHC protocol for TBR1 antibody 20932-1-AP | Download protocol |
| IF protocol for TBR1 antibody 20932-1-AP | Download protocol |
| IP protocol for TBR1 antibody 20932-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Neurosci A tau homeostasis signature is linked with the cellular and regional vulnerability of excitatory neurons to tau pathology. | ||
Nat Commun Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes. | ||
Nat Commun Disrupted neuronal maturation in Angelman syndrome-derived induced pluripotent stem cells. | ||
Proc Natl Acad Sci U S A Human intermediate progenitor diversity during cortical development. | ||
Cereb Cortex N-Methyl d-Aspartate Receptor Expression Patterns in the Human Fetal Cerebral Cortex. |
