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BCL11B Polyclonal antibody

BCL11B Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 55414-1-AP

Print datasheet

Synonyms

B cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B, BCL11B, COUP TF interacting protein 2, CTIP 2, CTIP2, hRit1, hRIT1 alpha, RIT1

验证数据展示

  • WB analysis using 55414-1-AP

    WB analysis using 55414-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 55414-1-AP

    IP experiment of Jurkat using 55414-1-AP

    IP result of anti-BCL11B (IP:55414-1-AP, 4ug; Detection:55414-1-AP 1:500) with Jurkat cells lysate 2800ug.

  • IHC staining of mouse brain using 55414-1-AP

    IHC staining of mouse brain using 55414-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55414-1-AP (BCL11B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 55414-1-AP

    IF Staining of HepG2 using 55414-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 55414-1-AP (BCL11B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, mouse thymus tissue, MOLT-4 cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55414-1-AP targets BCL11B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell CLL/lymphoma 11B (zinc finger protein)
Synonyms B cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B, BCL11B, COUP TF interacting protein 2, CTIP 2, CTIP2, hRit1, hRIT1 alpha, RIT1
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberNM_022898
Gene Symbol BCL11B
Gene ID (NCBI) 64919
RRIDAB_11182609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0K0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

实验方案

Product Specific Protocols
WB protocol for BCL11B antibody 55414-1-APDownload protocol
IHC protocol for BCL11B antibody 55414-1-APDownload protocol
IF protocol for BCL11B antibody 55414-1-APDownload protocol
IP protocol for BCL11B antibody 55414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Arch Virol

Increased expression of BCL11B and its recruited chromatin remodeling factors during highly active antiretroviral therapy synergistically represses the transcription of human immunodeficiency virus type 1 and is associated with residual immune activation.

Authors - Juan Wang
View Article