×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 10366-1-AP

    WB analysis using 10366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10366-1-AP (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 10366-1-AP

    IHC staining of human liver cancer using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Vimentin antibody included
  • IF Staining of hiPSC-derived cortical astrocytes (7 DIV) using 10366-1-AP

    IF Staining of hiPSC-derived cortical astrocytes (7 DIV) using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed BrainXell hiPSC-derived cortical astrocytes (7 DIV) using antibodies against GFAP and Vimentin (10366-1-AP) at a dilution of 1:200, 1:400, or 1:500. Data provided by BrainXell. ​  iPSC-Derived Human Neurons and Glia from BrainXell - BrainXell

  • WB analysis of Jurkat cells using 10366-1-AP

    WB analysis of Jurkat cells using 10366-1-AP

    WB result of Vimentin antibody (10366-1-AP, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

  • IF Staining of human breast cancer using 10366-1-AP

    IF Staining of human breast cancer using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human kidney using 10366-1-AP

    IF Staining of human kidney using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 10366-1-AP

    IF Staining of mouse brain using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10366-1-AP (Vimentin antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 10366-1-AP

    IF Staining of HepG2 using 10366-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10366-1-AP (Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 10366-1-AP

    FC experiment of HeLa using 10366-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Vimentin (10366-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of human breast cancer using 10366-1-AP

    IF Staining of human breast cancer using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 10366-1-AP

    IHC staining of human colon using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded normal colon slide using 10366-1-AP (Vimentin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Vimentin antibody included
  • IHC staining of human hepatocirrhosis using 10366-1-AP

    IHC staining of human hepatocirrhosis using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Vimentin antibody included
  • IF Staining of HepG2 using 10366-1-AP

    IF Staining of HepG2 using 10366-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (10366-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of mMSCs using 10366-1-AP

    FC experiment of mMSCs using 10366-1-AP

    1x10^6 mMSCs cells were intracellularly stained with 0.5 ug Vimentin Polyclonal antibody (10366-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA
Cat No. 10366-1-AP

产品说明书

引用文献(2612)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (6)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA and More (6)

别名

VIM

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human normal colon, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue, human kidney tissue
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells, hiPSC-derived cortical astrocytes (7 DIV)
Positive FC (Intra) detected inHeLa cells, mMSCs cells
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10366-1-AP targets Vimentin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, IP-MS, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA Application Description
文献引用应用IHC, IF, FC (Intra), IP, CoIP, RIP, IP-MS, ELISA, Cell treatment
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, rabbit, monkey, chicken, goat, sheep, deer
免疫原

CatNo: Ag0489

Product name: Recombinant human Vimentin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-466 aa of BC000163

Sequence: MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vimentin
别名 VIM
计算分子量 466 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC000163
基因名称 VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
RRIDAB_2273020
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08670
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

实验方案

Product Specific Protocols
FC protocol for Vimentin antibody 10366-1-APDownload protocol
IF protocol for Vimentin antibody 10366-1-APDownload protocol
IHC protocol for Vimentin antibody 10366-1-APDownload protocol
WB protocol for Vimentin antibody 10366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

iPEX enables micrometre-resolution deep spatial proteomics via tissue expansion.

Authors - Fengxiang Wang
View Article
humanWB

J Hematol Oncol

LincK contributes to breast tumorigenesis by promoting proliferation and epithelial-to-mesenchymal transition.

Authors - Jing Li
View Article
mouseIF

J Hematol Oncol

Combine and conquer: manganese synergizing anti-TGF-β/PD-L1 bispecific antibody YM101 to overcome immunotherapy resistance in non-inflamed cancers.

Authors - Ming Yi
View Article
humanFC (Intra)

Cell

Early rhombic lip Protogenin+ve stem cells in a human-specific neurovascular niche initiate and maintain group 3 medulloblastoma

Authors - Abhirami Visvanathan
View Article
humanIF

Cell

A transcription factor atlas of directed differentiation

Authors - Julia Joung
View Article
humanIHC

Mol Cancer

circNOX4 activates an inflammatory fibroblast niche to promote tumor growth and metastasis in NSCLC via FAP/IL-6 axis

Authors - Yan Zhao
View Article
||
New chat

Able™

正在加载，请稍候...