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Validation Data Gallery

  • Purity of recombinant human FGFbasic-TS was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGFbasic-TS was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

    Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

  • iPSC-derived Cardiomyocytes Cultured using Recombinant Human Activin A, BMP-4 and bFGF/FGF-2). iPSCs differentiated to Cardiomyocytes using HumanKine® recombinant human Activin A protein (HZ-1138), HumanKine® recombinant human BMP-4 protein (HZ-1045), HumanKine® recombinant human bFGF/FGF-2 (HZ-1285), were fixed and permeabilized for detection of endogenous Alpha actinin and Cardiac Troponin T using Alpha Actinin Polyclonal Antibody (Product # 11313-2-AP, 1:200 dilution, labelled with CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4)) and Cardiac Troponin T Polyclonal Antibody (Product # 15513-1-AP, 1:200 dilution, labelled with CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)).

    iPSC-derived Cardiomyocytes Cultured using Recombinant Human Activin A, BMP-4 and bFGF/FGF-2). iPSCs differentiated to Cardiomyocytes using HumanKine® recombinant human Activin A protein (HZ-1138), HumanKine® recombinant human BMP-4 protein (HZ-1045), HumanKine® recombinant human bFGF/FGF-2 (HZ-1285), were fixed and permeabilized for detection of endogenous Alpha actinin and Cardiac Troponin T using Alpha Actinin Polyclonal Antibody (Product # 11313-2-AP, 1:200 dilution, labelled with CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4)) and Cardiac Troponin T Polyclonal Antibody (Product # 15513-1-AP, 1:200 dilution, labelled with CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)).

  • Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF basic-TS for 72hrs in defined medium. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.4-2.5 ng/mL.

    Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF basic-TS for 72hrs in defined medium. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.4-2.5 ng/mL.

  • Proteintech FGF basic-TS (HZ-1285) demonstrates greater induction of proliferation and 3-fold lower EC50 compared to leading competitors. Recombinant human FGFbasic-TS stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

    Proteintech FGF basic-TS (HZ-1285) demonstrates greater induction of proliferation and 3-fold lower EC50 compared to leading competitors. Recombinant human FGFbasic-TS stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

  • Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

    Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGFbasic-TS protein

Cat No. HZ-1285
引用文献(100)

Activity

0.4-2.5 ng/mL in NIH3T3 cells in defined media

Specific Activity

minimally 6.00 x 106 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

Product type:  Research use only
规格价格库存

GMP-Grade HumanKine® Info

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Technical Specifications

GeneID 2247
Species Human
Expression HEK293
Activity 0.4-2.5 ng/mL in NIH3T3 cells in defined media
Specific Activityminimally 6.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP09038
Molecular Mass 17 kDa reduced and non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).Gently swirl or tap vial to mix.

Background

Basic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGFbasic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGFbasic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

Publications

SpeciesTitle
human

Nature

C-terminal amides mark proteins for degradation via SCF-FBXO31.

Authors - Matthias F. Muhar
View Article
human

Nat Biotechnol

An in vivo model of functional and vascularized human brain organoids.

Authors - Abed AlFatah Mansour
View Article
human

Cell Res

Visualization of aging-associated chromatin alterations with an engineered TALE system.

Authors - Ruotong Ren
View Article

Gastroenterology

BRD9-SMAD2/3 orchestrates stemness and tumorigenesis in pancreatic ductal adenocarcinoma

Authors - Yuliang Feng
View Article
human

Cell Stem Cell

FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration.

Authors - Pengze Yan
View Article
human

Cell Stem Cell

A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.

Authors - Haibin Xi
View Article
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