CoraLite® Plus 750-conjugated TGFB1 Recombinant antibody

TGFB1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-81746-2

产品说明书

CloneNo. 230544B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra)

TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide, TGF beta

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive WB detected in4T1 cells, RAW 264.7 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81746-2-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81746-2 targets TGFB1 in WB, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 TGFB1 fusion protein Ag13591 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor, beta 1
别名 TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide, TGF beta
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC000125
基因名称 TGFB1
Gene ID (NCBI) 7040
RRIDAB_3673734
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01137
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 TGFB1 antibody CL750-81746-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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