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0
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验证数据展示

  • WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

    WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81746-2-RR (same clone as 81746-2-PBS)

    WB analysis of HepG2 using 81746-2-RR (same clone as 81746-2-PBS)

    WB result of TGF Beta 1 antibody (81746-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGF Beta 1 transfected HepG2 cells. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TGF beta 1 antibody included
  • IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TGF beta 1 antibody included
  • IF Staining of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    IF Staining of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGFB1 antibody (81746-2-RR, Clone: 230544B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    FC experiment of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug TGF Beta 1 Recombinant antibody (81746-2-RR, Clone:230544B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81746-2-RR

    Affinity and Kinetic Characterization of 81746-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81746-2-RR against Human TGFB1 were performed. The affinity constant is 9.65 nM.

  • Featured Product
  • KD/KO验证

TGF Beta 1 Recombinant antibody, PBS Only

TGF Beta 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81746-2-PBS

产品说明书

CloneNo. 230544B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.

产品信息

81746-2-PBS targets TGF Beta 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TGF Beta 1 fusion protein Ag13591 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor, beta 1
别名 TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC000125
基因名称 TGFB1
Gene ID (NCBI) 7040
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01137
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1). Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).