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Species Reactivity
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Species Reactivity
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Conjugate
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0
Conjugate
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验证数据展示

  • WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

    WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81746-2-RR (same clone as 81746-2-PBS)

    WB analysis of HepG2 using 81746-2-RR (same clone as 81746-2-PBS)

    WB result of TGF Beta 1 antibody (81746-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGF Beta 1 transfected HepG2 cells. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TGF beta 1 antibody included
  • IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TGF beta 1 antibody included
  • IF Staining of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    IF Staining of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGFB1 antibody (81746-2-RR, Clone: 230544B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    FC experiment of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug TGF Beta 1 Recombinant antibody (81746-2-RR, Clone:230544B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81746-2-RR

    Affinity and Kinetic Characterization of 81746-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81746-2-RR against Human TGFB1 were performed. The affinity constant is 9.65 nM.

  • Featured Product
  • KD/KO验证

TGF Beta 1 Recombinant antibody, PBS Only

TGF Beta 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81746-2-PBS

产品说明书

CloneNo. 230544B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.

产品信息

81746-2-PBS targets TGF Beta 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TGF Beta 1 fusion protein Ag13591 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor, beta 1
别名 TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC000125
基因名称 TGFB1
Gene ID (NCBI) 7040
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01137
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.