验证数据展示
经过测试的应用
Positive IF/ICC detected in | HEK-293 cells |
Positive FC (Intra) detected in | PHA treated human PBMCs, HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
PE-81746-2 targets TGF Beta 1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human, mouse |
免疫原 | TGF Beta 1 fusion protein Ag13591 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | transforming growth factor, beta 1 |
别名 | TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide |
计算分子量 | 44 kDa |
观测分子量 | 44 kDa |
GenBank蛋白编号 | BC000125 |
基因名称 | TGFB1 |
Gene ID (NCBI) | 7040 |
偶联类型 | PE Fluorescent Dye |
最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P01137 |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.
实验方案
Product Specific Protocols | |
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IF protocol for PE TGF Beta 1 antibody PE-81746-2 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |