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验证数据展示

  • IF Staining of MCF-7 using CL488-85884-5

    IF Staining of MCF-7 using CL488-85884-5

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 RBBP6 antibody (CL488-85884-5, Clone: 250142C1 ) at dilution of 1:200, CL594-Phalloidin (red).

  • IF Staining of Caco-2 using CL488-85884-5

    IF Staining of Caco-2 using CL488-85884-5

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 RBBP6 antibody (CL488-85884-5, Clone: 250142C1 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated RBBP6 Recombinant monoclonal antibody

RBBP6 Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-85884-5

产品说明书

CloneNo. 250142C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inCaco-2 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RBBP6 Ready-To-Use IHC Kit. RBBP6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 85884-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-85884-5 targets RBBP6 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag2484

Product name: Recombinant human RBBP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC029352

Sequence: MSCVHYKFSSKLNYDTVTFDGLHISLCDLKKQIMGREKLKAADCDLQITNAQTKEEYTDDNALIPKNSSVIVRRIPIGGVKSTSKTYVISRTEPAMATTKAVCKNTISHFFYTLLLPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoblastoma binding protein 6
别名 E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR
计算分子量 1792 aa, 200 kDa
观测分子量250 kDa
GenBank蛋白编号BC029352
基因名称 RBBP6
Gene ID (NCBI) 5930
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z6E9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RBBP6 antibody CL488-85884-5Download protocol
Standard Protocols
Click here to view our Standard Protocols
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