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验证数据展示

  • WB analysis using 85884-5-RR

    WB analysis using 85884-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85884-5-RR (RBBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 85884-5-RR

    IF Staining of MCF-7 using 85884-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RBBP6 antibody (85884-5-RR, Clone: 250142C1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RBBP6 Recombinant antibody

RBBP6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85884-5-RR

产品说明书

CloneNo. 250142C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEC109 cells, NIH/3T3 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RBBP6 Ready-To-Use IHC Kit. RBBP6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85884-5-RR targets RBBP6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RBBP6 fusion protein Ag2484 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoblastoma binding protein 6
别名 E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR
计算分子量 1792 aa, 200 kDa
观测分子量250 kDa
GenBank蛋白编号BC029352
基因名称 RBBP6
Gene ID (NCBI) 5930
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z6E9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

实验方案

Product Specific Protocols
WB protocol for RBBP6 antibody 85884-5-RRDownload protocol
IF protocol for RBBP6 antibody 85884-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols