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验证数据展示

  • IF Staining of U2OS using CL488-82875

    IF Staining of U2OS using CL488-82875

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 SHPK antibody (CL488-82875, Clone: 230115A4 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-82875

    FC experiment of U2OS using CL488-82875

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 SHPK Recombinant Antibody (CL488-82875, Clone:230115A4)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated SHPK Recombinant monoclonal antibody

SHPK Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82875

产品说明书

CloneNo. 230115A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

230115A4, Carbohydrate kinase-like protein, CARKL, EC:2.7.1.14, Sedoheptulokinase

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 82875-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82875 targets SHPK in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag33893

Product name: Recombinant human SHPK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 65-253 aa of BC020543

Sequence: ILQALHECLAALPRPQLRSVVGIGVSGQMHGVVFWKTGQGCEWTEGGITPVFEPRAVSHLVTWQDGRCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sedoheptulokinase
别名 230115A4, Carbohydrate kinase-like protein, CARKL, EC:2.7.1.14, Sedoheptulokinase
计算分子量 478 aa, 52 kDa
GenBank蛋白编号BC020543
基因名称 SHPK
Gene ID (NCBI) 23729
RRIDAB_3673091
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHJ6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SHPK antibody CL488-82875Download protocol
Standard Protocols
Click here to view our Standard Protocols
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