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验证数据展示

  • IHC staining of Human Hepatocellular cancer using 82875-1-RR

    IHC staining of Human Hepatocellular cancer using 82875-1-RR

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82875-1-RR (SHPK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of Human Hepatocellular cancer using 82875-1-RR

    IHC staining of Human Hepatocellular cancer using 82875-1-RR

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82875-1-RR (SHPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 82875-1-RR

    IF Staining of U2OS using 82875-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SHPK antibody (82875-1-RR, Clone: 230115A4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of U-2 OS using 82875-1-RR

    FC experiment of U-2 OS using 82875-1-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human SHPK (82875-1-RR, Clone:230115A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82875-1-RR

    Affinity and Kinetic Characterization of 82875-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82875-1-RR against Human SHPK were performed. The affinity constant is 18.1 pM.

SHPK Recombinant monoclonal antibody

SHPK Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82875-1-RR

产品说明书

CloneNo. 230115A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

230115A4, Carbohydrate kinase-like protein, CARKL, EC:2.7.1.14, Sedoheptulokinase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inHuman Hepatocellular cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-2 OS

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82875-1-RR targets SHPK in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33893

Product name: Recombinant human SHPK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 65-253 aa of BC020543

Sequence: ILQALHECLAALPRPQLRSVVGIGVSGQMHGVVFWKTGQGCEWTEGGITPVFEPRAVSHLVTWQDGRCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sedoheptulokinase
别名 230115A4, Carbohydrate kinase-like protein, CARKL, EC:2.7.1.14, Sedoheptulokinase
计算分子量 478 aa, 52 kDa
GenBank蛋白编号BC020543
基因名称 SHPK
Gene ID (NCBI) 23729
RRIDAB_3670594
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

实验方案

Product Specific Protocols
IF protocol for SHPK antibody 82875-1-RRDownload protocol
IHC protocol for SHPK antibody 82875-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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