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验证数据展示

  • IHC staining of Human Hepatocellular cancer using 82875-1-RR (same clone as 82875-1-PBS)

    IHC staining of Human Hepatocellular cancer using 82875-1-RR (same clone as 82875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82875-1-RR (SHPK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82875-1-PBS in a different storage buffer formulation.

  • IHC staining of Human Hepatocellular cancer using 82875-1-RR (same clone as 82875-1-PBS)

    IHC staining of Human Hepatocellular cancer using 82875-1-RR (same clone as 82875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82875-1-RR (SHPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82875-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82875-1-RR (same clone as 82875-1-PBS)

    IF Staining of U2OS using 82875-1-RR (same clone as 82875-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SHPK antibody (82875-1-RR, Clone: 230115A4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82875-1-PBS in a different storage buffer formulation.

  • FC experiment of U-2 OS using 82875-1-RR (same clone as 82875-1-PBS)

    FC experiment of U-2 OS using 82875-1-RR (same clone as 82875-1-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human SHPK (82875-1-RR, Clone:230115A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82875-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82875-1-RR

    Affinity and Kinetic Characterization of 82875-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82875-1-RR against Human SHPK were performed. The affinity constant is 18.1 pM.

SHPK Recombinant monoclonal antibody, PBS Only

SHPK Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82875-1-PBS

产品说明书

CloneNo. 230115A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

230115A4, Carbohydrate kinase-like protein, CARKL, EC:2.7.1.14, Sedoheptulokinase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82875-1-PBS targets SHPK in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33893

Product name: Recombinant human SHPK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 65-253 aa of BC020543

Sequence: ILQALHECLAALPRPQLRSVVGIGVSGQMHGVVFWKTGQGCEWTEGGITPVFEPRAVSHLVTWQDGRCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sedoheptulokinase
别名 230115A4, Carbohydrate kinase-like protein, CARKL, EC:2.7.1.14, Sedoheptulokinase
计算分子量 478 aa, 52 kDa
GenBank蛋白编号BC020543
基因名称 SHPK
Gene ID (NCBI) 23729
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHJ6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

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