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验证数据展示

  • IF Staining of MCF-7 using CL488-85837-3

    IF Staining of MCF-7 using CL488-85837-3

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 SENP3 antibody (CL488-85837-3, Clone: 250153A9 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated SENP3 Recombinant monoclonal antibody

SENP3 Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-85837-3

产品说明书

CloneNo. 250153A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

EC:3.4.22.-, Sentrin/SUMO-specific protease SENP3, Sentrin-specific protease 3, SMT3IP1, SSP3

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SENP3 Ready-To-Use IHC Kit. SENP3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 85837-3-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-85837-3 targets SENP3 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11911

Product name: Recombinant human SENP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-574 aa of BC080658

Sequence: GLRWTPKSPLDPDSGLLSCTLPNGFGGQSGPEGERSLAPPDASILISNVCSIGDHVAQELFQGSDLGMAEEAERPGEKAGQHSPLREEHVTCVQSILDEFLQTYGSLIPLSTDEVVEKLEDIFQQEFSTPSRKGLVLQLIQSYQRMPGNAMVRGFRVAYKRHVLTMDDLGTLYGQNWLNDQVMNMYGDLVMDTVPEKVHFFNSFFYDKLRTKGYDGVKRWTKNVDIFNKELLLIPIHLEVHWSLISVDVRRRTITYFDSQRTLNRRCPKHIAKYLQAEAVKKDRLDFHQGWKGYFKMNVARQNNDSDCGAFVLQYCKHLALSQPFSFTQQDMPKLRRQIYKELCHCKLTV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SUMO1/sentrin/SMT3 specific peptidase 3
别名 EC:3.4.22.-, Sentrin/SUMO-specific protease SENP3, Sentrin-specific protease 3, SMT3IP1, SSP3
计算分子量 574 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC080658
基因名称 SENP3
Gene ID (NCBI) 26168
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H4L4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SENP3 antibody CL488-85837-3Download protocol
Standard Protocols
Click here to view our Standard Protocols
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