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验证数据展示

  • WB analysis using 85837-3-RR (same clone as 85837-3-PBS)

    WB analysis using 85837-3-RR (same clone as 85837-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85837-3-RR (SENP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85837-3-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 85837-3-RR (same clone as 85837-3-PBS)

    IF Staining of MCF-7 using 85837-3-RR (same clone as 85837-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SENP3 antibody (85837-3-RR, Clone: 250153A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85837-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02141-2

    Cytometric bead array standard curve of MP02141-2

    Cytometric bead array standard curve of MP02141-2, SENP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85837-3-PBS. Detection antibody: 85837-2-PBS. Standard: Ag11911. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP02141-1

    Cytometric bead array standard curve of MP02141-1

    Cytometric bead array standard curve of MP02141-1, SENP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85837-3-PBS. Detection antibody: 85837-1-PBS. Standard: Ag11911. Range: 0.781-50 ng/mL.

  • Affinity and Kinetic Characterization of 85837-3-RR

    Affinity and Kinetic Characterization of 85837-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85837-3-RR against Human SENP3 were performed. The affinity constant is 0.578 nM.

SENP3 Recombinant antibody, PBS Only (Capture)

SENP3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85837-3-PBS

产品说明书

CloneNo. 250153A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

EC:3.4.22.-, Sentrin/SUMO-specific protease SENP3, Sentrin-specific protease 3, SMT3IP1, SSP3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85837-3-PBS targets SENP3 as part of a matched antibody pair:

MP02141-1: 85837-3-PBS capture and 85837-1-PBS detection (validated in Cytometric bead array)

MP02141-2: 85837-3-PBS capture and 85837-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SENP3 fusion protein Ag11911 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SUMO1/sentrin/SMT3 specific peptidase 3
别名 EC:3.4.22.-, Sentrin/SUMO-specific protease SENP3, Sentrin-specific protease 3, SMT3IP1, SSP3
计算分子量 574 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC080658
基因名称 SENP3
Gene ID (NCBI) 26168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H4L4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.