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验证数据展示

  • WB analysis using 85837-3-RR

    WB analysis using 85837-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85837-3-RR (SENP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 85837-3-RR

    IF Staining of MCF-7 using 85837-3-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SENP3 antibody (85837-3-RR, Clone: 250153A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85837-3-RR

    Affinity and Kinetic Characterization of 85837-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85837-3-RR against Human SENP3 were performed. The affinity constant is 0.578 nM.

SENP3 Recombinant antibody

SENP3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85837-3-RR

产品说明书

CloneNo. 250153A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

EC:3.4.22.-, Sentrin/SUMO-specific protease SENP3, Sentrin-specific protease 3, SMT3IP1, SSP3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Caco-2 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85837-3-RR targets SENP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SENP3 fusion protein Ag11911 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SUMO1/sentrin/SMT3 specific peptidase 3
别名 EC:3.4.22.-, Sentrin/SUMO-specific protease SENP3, Sentrin-specific protease 3, SMT3IP1, SSP3
计算分子量 574 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC080658
基因名称 SENP3
Gene ID (NCBI) 26168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H4L4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.

实验方案

Product Specific Protocols
WB protocol for SENP3 antibody 85837-3-RRDownload protocol
IF protocol for SENP3 antibody 85837-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols