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验证数据展示

  • WB analysis using 86307-1-RR

    WB analysis using 86307-1-RR

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • WB analysis using 86307-1-RR

    WB analysis using 86307-1-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • IF Staining of Hela using 86307-1-RR

    IF Staining of Hela using 86307-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated Hela cells using Phospho-Cofilin (Ser3) antibody (86307-1-RR, Clone: 250986H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-Cofilin (Ser3) Recombinant monoclonal antibody

Phospho-Cofilin (Ser3) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86307-1-RR

产品说明书

CloneNo. 250986H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Cofilin (phospho S3), Cofilin (Ser3), p Cofilin, p-Cofilin, p-Cofilin (Ser3)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, λ phosphatase treated HeLa cells, λ phosphatase treated NIH/3T3 cells
Positive IF/ICC detected inλ phosphatase treated Hela cells
Planning an IHC experiment? We recommend our IHCeasy CFL1 Ready-To-Use IHC Kit. CFL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86307-1-RR targets Phospho-Cofilin (Ser3) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cofilin 1 (non-muscle)
别名 Cofilin (phospho S3), Cofilin (Ser3), p Cofilin, p-Cofilin, p-Cofilin (Ser3)
计算分子量 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC012318
基因名称 Cofilin
Gene ID (NCBI) 1072
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23528
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

实验方案

Product Specific Protocols
IF protocol for Phospho-Cofilin (Ser3) antibody 86307-1-RRDownload protocol
WB protocol for Phospho-Cofilin (Ser3) antibody 86307-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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