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验证数据展示

  • WB analysis using 86307-1-RR (same clone as 86307-1-PBS)

    WB analysis using 86307-1-RR (same clone as 86307-1-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

  • WB analysis using 86307-1-RR (same clone as 86307-1-PBS)

    WB analysis using 86307-1-RR (same clone as 86307-1-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

  • IF Staining of Hela using 86307-1-RR (same clone as 86307-1-PBS)

    IF Staining of Hela using 86307-1-RR (same clone as 86307-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-Cofilin (Ser3) antibody (86307-1-RR, Clone: 250986H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

Phospho-Cofilin (Ser3) Recombinant monoclonal antibody, PBS Only

Phospho-Cofilin (Ser3) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86307-1-PBS

产品说明书

CloneNo. 250986H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Cofilin (phospho S3), Cofilin (Ser3), p Cofilin, p-Cofilin, p-Cofilin (Ser3)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CFL1 Ready-To-Use IHC Kit. CFL1 primary antibody included.

产品信息

86307-1-PBS targets Phospho-Cofilin (Ser3) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cofilin 1 (non-muscle)
别名 Cofilin (phospho S3), Cofilin (Ser3), p Cofilin, p-Cofilin, p-Cofilin (Ser3)
计算分子量 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC012318
基因名称 Cofilin
Gene ID (NCBI) 1072
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23528
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

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