验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue |
For other applications, we recommend the unconjugated version of this antibody, 86370-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-86370 targets Pan-Keratin (TypeⅡ) in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Pan-Keratin (TypeⅡ) |
| 别名 | Pan Keratin, Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins |
| 观测分子量 | 52-68 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14533 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Pan-Keratin (TypeⅡ) antibody CL488-86370 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
