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验证数据展示

  • WB analysis using 86370-1-RR

    WB analysis using 86370-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 86370-1-RR

    IHC staining of human stomach cancer using 86370-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 86370-1-RR

    IHC staining of human stomach cancer using 86370-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human colon cancer using 86370-1-RR

    IF Staining of human colon cancer using 86370-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Pan-Keratin (TypeⅡ) Recombinant antibody

Pan-Keratin (TypeⅡ) Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86370-1-RR

产品说明书

CloneNo. 251040F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins, Type II keratin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HT-29 cells, mouse skin tissue, rat colon tissue, rat skin tissue
Positive IHC detected inhuman stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue, human cervical cancer tissue, human lung cancer tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86370-1-RR targets Pan-Keratin (TypeⅡ) in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Pan-Keratin (TypeⅡ)
别名 Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins, Type II keratin
观测分子量52-68 kDa
基因名称
Gene ID (NCBI) Pan-Keratin (TypeⅡ)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86.

实验方案

Product Specific Protocols
WB protocol for Pan-Keratin (TypeⅡ) antibody 86370-1-RRDownload protocol
IHC protocol for Pan-Keratin (TypeⅡ) antibody 86370-1-RRDownload protocol
IF protocol for Pan-Keratin (TypeⅡ) antibody 86370-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols