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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86370-1-RR (same clone as 86370-1-PBS)

    WB analysis using 86370-1-RR (same clone as 86370-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

    IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

    IHC staining of human stomach cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 86370-1-RR (same clone as 86370-1-PBS)

    IF Staining of human colon cancer using 86370-1-RR (same clone as 86370-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86370-1-PBS in a different storage buffer formulation.

Pan-Keratin (TypeⅡ) Recombinant antibody, PBS Only

Pan-Keratin (TypeⅡ) Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86370-1-PBS

产品说明书

CloneNo. 251040F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins, Type II keratin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86370-1-PBS targets Pan-Keratin (TypeⅡ) in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Pan-Keratin (TypeⅡ)
别名 Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins, Type II keratin
观测分子量52-68 kDa
基因名称
Gene ID (NCBI) Pan-Keratin (TypeⅡ)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14533
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86.