验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84657-5 targets PSPC1 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10136 Product name: Recombinant human PSPC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC014184 Sequence: MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | paraspeckle component 1 |
| 别名 | 242158C1, PSP1, PSPC 1 |
| 计算分子量 | 523 aa, 59 kDa |
| GenBank蛋白编号 | BC014184 |
| 基因名称 | PSPC1 |
| Gene ID (NCBI) | 55269 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8WXF1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Paraspeckle component 1 (PSPC1) is a paranuclear spot protein, which is known as a paraspeckle biomarker and is a putative transcription factor that belongs to the Drosophila behavior/human splicing family with limited knowledge about its role in cancers. High PSPC1 expression in cancer tissues compared to corresponding benign tissues is associated with late tumor stages in patients with cancer and poor patient survival in breast, lung, and liver cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PSPC1 antibody CL488-84657-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
