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验证数据展示

  • IF Staining of HepG2 using 84657-5-RR

    IF Staining of HepG2 using 84657-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSPC1 antibody (84657-5-RR, Clone: 242158C1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 84657-5-RR

    IF Staining of HepG2 using 84657-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSPC1 antibody (84657-5-RR, Clone: 242158C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PSPC1 Recombinant monoclonal antibody

PSPC1 Uni-rAb® Recombinant Antibody for IF/ICC, ELISA
Cat No. 84657-5-RR

产品说明书

CloneNo. 242158C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

242158C1, PSP1, PSPC 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSPC1 Ready-To-Use IHC Kit. PSPC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84657-5-RR targets PSPC1 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10136

Product name: Recombinant human PSPC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC014184

Sequence: MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 paraspeckle component 1
别名 242158C1, PSP1, PSPC 1
计算分子量 523 aa, 59 kDa
GenBank蛋白编号BC014184
基因名称 PSPC1
Gene ID (NCBI) 55269
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8WXF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paraspeckle component 1 (PSPC1) is a paranuclear spot protein, which is known as a paraspeckle biomarker and is a putative transcription factor that belongs to the Drosophila behavior/human splicing family with limited knowledge about its role in cancers. High PSPC1 expression in cancer tissues compared to corresponding benign tissues is associated with late tumor stages in patients with cancer and poor patient survival in breast, lung, and liver cancers.

实验方案

Product Specific Protocols
IF protocol for PSPC1 antibody 84657-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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