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验证数据展示

  • FC experiment of HeLa using CL647-67817

    FC experiment of HeLa using CL647-67817

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human PSMA7 (CL647-67817, Clone:1F2C6) (red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated PSMA7 Monoclonal antibody

PSMA7 Monoclonal Antibody for FC (Intra)
Cat No. CL647-67817

产品说明书

CloneNo. 1F2C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

XAPC7, RC6 1, Proteasome subunit RC6-1, Proteasome subunit alpha type-7, HSPC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMA7 Ready-To-Use IHC Kit. PSMA7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67817-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67817 targets PSMA7 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7821

Product name: Recombinant human PSMA7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-248 aa of BC004427

Sequence: MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 7
别名 XAPC7, RC6 1, Proteasome subunit RC6-1, Proteasome subunit alpha type-7, HSPC
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC004427
基因名称 PSMA7
Gene ID (NCBI) 5688
RRIDAB_3673646
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDO14818
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA7(Proteasome subunit alpha type-7) is also named as HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis.(PMID: 19442227).