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验证数据展示

  • WB analysis using CL488-67817

    WB analysis using CL488-67817

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67817 (PSMA7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using CL488-67817

    IF Staining of MCF-7 using CL488-67817

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PSMA7 antibody (CL488-67817, Clone: 1F2C6 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PSMA7 Monoclonal antibody

PSMA7 Monoclonal Antibody for WB, IF/ICC
Cat No. CL488-67817

产品说明书

CloneNo. 1F2C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC

别名

C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA7 Ready-To-Use IHC Kit. PSMA7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67817-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67817 targets PSMA7 in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7821

Product name: Recombinant human PSMA7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-248 aa of BC004427

Sequence: MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 7
别名 C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC004427
基因名称 PSMA7
Gene ID (NCBI) 5688
RRIDAB_3672964
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDO14818
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA7(Proteasome subunit alpha type-7) is also named as HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis.(PMID: 19442227).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 PSMA7 antibody CL488-67817Download protocol
Standard Protocols
Click here to view our Standard Protocols
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