验证数据展示
经过测试的应用
Positive FC (Intra) detected in | mouse peritoneal macrophages |
Positive FC detected in | mouse peritoneal macrophages |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
FITC-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with Mouse samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | Mouse |
免疫原 | CHO expressed, recombinant mouse IL-12 p70 种属同源性预测 |
宿主/亚型 | Rat / IgG2a, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | interleukin 12b |
别名 | Il 12b, Il 12p40, Il12b, Il12p40, interleukin 12b, p40 |
GenBank蛋白编号 | BC103608 |
基因名称 | Il12b |
Gene ID (NCBI) | 16160 |
RRID | AB_3084997 |
偶联类型 | FITC Plus Fluorescent Dye |
最大激发/发射波长 | 495 nm / 524 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).
实验方案
Product Specific Protocols | |
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FC protocol for FITC Plus IL-12/IL-23 p40 antibody FITC-65097 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |