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验证数据展示

  • FC experiment of mouse peritoneal macrophages using CL647-65097

    FC experiment of mouse peritoneal macrophages using CL647-65097

    1X10^6 IFN-γ primed murine peritoneal macrophages stimulated with LPS were surface stained with PE Anti-Mouse CD11b (M1/70), then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (CL647-65097, Clone: C17.8) or 0.5 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3).

CoraLite® Plus 647 Anti-Mouse IL-12/IL-23 p40 (C17.8)

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)
Cat No. CL647-65097

产品说明书

CloneNo. C17.8

宿主/亚型

Rat / IgG2a, kappa

种属反应性

mouse

应用

FC (Intra)

别名

Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inIFN-γ mouse peritoneal macrophages
For other applications, we recommend the unconjugated version of this antibody, 65097-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 CHO expressed, recombinant mouse IL-12 p70 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 12b
别名 Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b
GenBank蛋白编号BC103608
基因名称 Il12b
Gene ID (NCBI) 16160
RRIDAB_2920255
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IL-12/IL-23 p40 antibody CL647-65097Download protocol
Standard Protocols
Click here to view our Standard Protocols