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CoraLite®594 Anti-Mouse IL-12/IL-23 p40 (C17.8)

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

C17.8

Cat No : CL594-65097

Print datasheet

Synonyms

Il 12b, Il 12p40, Il12b, Il12p40, interleukin 12b, p40

验证数据展示

  • FC experiment of mouse peritoneal macrophages using CL594-65097

    FC experiment of mouse peritoneal macrophages using CL594-65097

    1X10^6 IFN-γ primed Balb/c mouse peritoneal macrophages were unstimulated or stimulated with LPS in the presence of a protein transport inhibitor and then intracellularly stained with 0.5 ug CoraLite®594 Anti-Mouse IL-12/IL-23 p40 (CL594-65097, Clone:C17.8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inmouse peritoneal macrophages
For other applications, we recommend the unconjugated version of this antibody, 65097-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with Mouse samples.

Tested Applications FC (Intra)
Tested Reactivity Mouse
Immunogen CHO expressed, recombinant mouse IL-12 p70 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 12b
Synonyms Il 12b, Il 12p40, Il12b, Il12p40, interleukin 12b, p40
GenBank Accession NumberBC103608
Gene Symbol Il12b
Gene ID (NCBI) 16160
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

实验方案

Product Specific Protocols
FC protocol for CL594 IL-12/IL-23 p40 antibody CL594-65097Download protocol
Standard Protocols
Click here to view our Standard Protocols