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验证数据展示

  • WB analysis using 86378-1-RR (same clone as 86378-1-PBS)

    WB analysis using 86378-1-RR (same clone as 86378-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86378-1-RR (GLDC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86378-1-PBS in a different storage buffer formulation.

  • WB analysis of Vero using 86378-1-RR (same clone as 86378-1-PBS)

    WB analysis of Vero using 86378-1-RR (same clone as 86378-1-PBS)

    Vero cells were subjected to SDS PAGE followed by western blot with 86378-1-RR (GLDC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86378-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 86378-1-RR (same clone as 86378-1-PBS)

    IHC staining of human liver using 86378-1-RR (same clone as 86378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86378-1-RR (GLDC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86378-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLDC antibody included
  • IHC staining of human liver using 86378-1-RR (same clone as 86378-1-PBS)

    IHC staining of human liver using 86378-1-RR (same clone as 86378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86378-1-RR (GLDC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86378-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLDC antibody included

GLDC Recombinant monoclonal antibody, PBS Only

GLDC Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86378-1-PBS

产品说明书

CloneNo. 250916A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, Indirect ELISA

别名

aminomethyl-transferring, EC:1.4.4.2, GCE, GCSP, Glycine dehydrogenase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® GLDC Ready-To-Use IHC Kit. GLDC primary antibody included.

产品信息

86378-1-PBS targets GLDC in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag20410

Product name: Recombinant human GLDC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 675-1020 aa of BC111995

Sequence: NIDAVHLKAMVDKHKENLAAIMITYPSTNGVFEENISDVCDLIHQHGGQVYLDGANMNAQVGICRPGDFGSDVSHLNLHKTFCIPHGGGGPGMGPIGVKKHLAPFLPNHPVISLKRNEDACPVGTVSAAPWGSSSILPISWAYIKMMGGKGLKQATETAILNANYMAKRLETHYRILFRGARGYVGHEFILDTRPFKKSANIEAVDVAKRLQDYGFHAPTMSWPVAGTLMVEPTESEDKAELDRFCDAMISIRQEIADIEEGRIDPRVNPLKMSPHSLTCVTSSHWDRPYSREVAAFPLPFVKPENKFWPTIARIDDIYGDQHLVCTCPPMEVYESPFSEQKRASS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycine dehydrogenase (decarboxylating)
别名 aminomethyl-transferring, EC:1.4.4.2, GCE, GCSP, Glycine dehydrogenase
计算分子量 1020 aa, 113 kDa
观测分子量 113 kDa
GenBank蛋白编号BC111995
基因名称 GLDC
Gene ID (NCBI) 2731
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23378
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLDC(Glycine dehydrogenase [decarboxylating], mitochondrial) is also named as GCSP and belongs to the GcvP family. It induces dramatic changes in glycolysis and glycine/serine metabolism that leads to changes in pyrimidine metabolism to regulate cancer cell proliferation(PMID:22225612).

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