×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86378-1-RR

    WB analysis using 86378-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86378-1-RR (GLDC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Vero using 86378-1-RR

    WB analysis of Vero using 86378-1-RR

    Vero cells were subjected to SDS PAGE followed by western blot with 86378-1-RR (GLDC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 86378-1-RR

    WB analysis of HepG2 using 86378-1-RR

    WB result of GLDC antibody (86378-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLDC transfected HepG2 cells.

  • IHC staining of human placenta using 86378-1-RR

    IHC staining of human placenta using 86378-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86378-1-RR (GLDC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLDC antibody included
  • IHC staining of rat kidney using 86378-1-RR

    IHC staining of rat kidney using 86378-1-RR

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 86378-1-RR (GLDC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLDC antibody included
  • IHC staining of rat liver using 86378-1-RR

    IHC staining of rat liver using 86378-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86378-1-RR (GLDC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLDC antibody included
  • IHC staining of rat spleen using 86378-1-RR

    IHC staining of rat spleen using 86378-1-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86378-1-RR (GLDC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLDC antibody included
  • IHC staining of human liver using 86378-1-RR

    IHC staining of human liver using 86378-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86378-1-RR (GLDC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLDC antibody included
  • IHC staining of human liver using 86378-1-RR

    IHC staining of human liver using 86378-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86378-1-RR (GLDC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLDC antibody included
  • KD/KO Validated

GLDC Recombinant monoclonal antibody

GLDC Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86378-1-RR

产品说明书

CloneNo. 250916A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, ELISA

别名

aminomethyl-transferring, EC:1.4.4.2, GCE, GCSP, Glycine dehydrogenase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, Vero cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, human placenta tissue, rat kidney tissue, rat liver tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® GLDC Ready-To-Use IHC Kit. GLDC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86378-1-RR targets GLDC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag20410

Product name: Recombinant human GLDC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 675-1020 aa of BC111995

Sequence: NIDAVHLKAMVDKHKENLAAIMITYPSTNGVFEENISDVCDLIHQHGGQVYLDGANMNAQVGICRPGDFGSDVSHLNLHKTFCIPHGGGGPGMGPIGVKKHLAPFLPNHPVISLKRNEDACPVGTVSAAPWGSSSILPISWAYIKMMGGKGLKQATETAILNANYMAKRLETHYRILFRGARGYVGHEFILDTRPFKKSANIEAVDVAKRLQDYGFHAPTMSWPVAGTLMVEPTESEDKAELDRFCDAMISIRQEIADIEEGRIDPRVNPLKMSPHSLTCVTSSHWDRPYSREVAAFPLPFVKPENKFWPTIARIDDIYGDQHLVCTCPPMEVYESPFSEQKRASS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycine dehydrogenase (decarboxylating)
别名 aminomethyl-transferring, EC:1.4.4.2, GCE, GCSP, Glycine dehydrogenase
计算分子量 1020 aa, 113 kDa
观测分子量 113 kDa
GenBank蛋白编号BC111995
基因名称 GLDC
Gene ID (NCBI) 2731
RRIDAB_3744850
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23378
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLDC(Glycine dehydrogenase [decarboxylating], mitochondrial) is also named as GCSP and belongs to the GcvP family. It induces dramatic changes in glycolysis and glycine/serine metabolism that leads to changes in pyrimidine metabolism to regulate cancer cell proliferation(PMID:22225612).

实验方案

Product Specific Protocols
IHC protocol for GLDC antibody 86378-1-RRDownload protocol
WB protocol for GLDC antibody 86378-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...