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CoraLite® Plus 488-conjugated FER Polyclonal antibody

FER Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-25287

Print datasheet

Synonyms

FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer

验证数据展示

  • FC experiment of Jurkat using CL488-25287

    FC experiment of Jurkat using CL488-25287

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FER (CL488-25287) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy FER Ready-To-Use IHC Kit. FER primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25287-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-25287 is FER Fusion Protein expressed in E. coli.

Tested Applications FC (Intra)
Tested Reactivity human, mouse
Immunogen FER fusion protein Ag18173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fer (fps/fes related) tyrosine kinase
Synonyms FER, p94 Fer, Proto oncogene c Fer, TYK3, Tyrosine kinase 3, Tyrosine protein kinase Fer
Calculated Molecular Weight 822 aa, 95 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC017060
Gene Symbol FER
Gene ID (NCBI) 2241
RRIDAB_2919202
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16591
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FER antibody CL488-25287Download protocol
Standard Protocols
Click here to view our Standard Protocols